Imaging brain activity deep in the cortex of freely moving mouse.
How does the brain produce behavior?
Studying the brain during behavior is most informative when the animal is free to interact with its environment in any way it chooses.
This requires small head-mounted devices that provide access to the brain, but that do not interfere with the animal’s behavior.
“We are interested in how freely behaving animals use vision to make decisions in their everyday life, and as many of the brain cells that are thought to be involved in this process are located deep in the visual cortex, we made a very light-weight head-mounted microscope that can measure activity from these neurons but does not interfere with the animal’s behavior.
We have made a large step towards finally imaging brain activity deep in the cortex of animals performing natural visual-based behaviors” said Jason Kerr, director of the Dept.
of Behavior and Brain Organization, Max Planck Institute for Neurobiology of Behavior (MPINB), Bonn, Germany.
Foreign [Music] Activity deep in the cortex of freely Moving mouse how does the brain produce Behavior studying the brain during Behavior is most informative when the Animal is free to interact with its Environment in any way it chooses this Requires small head mounted devices that Provide access to the brain but that do Not interfere with the animal's behavior We are interested in how freely behaving Animals use Vision to make decisions in Their everyday life and as many of the Brain cells that are thought to be Involved in this process are located Deep in the visual cortex we made a very Lightweight head mounted microscope that Can measure activity from these neurons But does not interfere with the animal's Behavior we have made a large step Towards finally Imaging brain activity Deep in the cortex of animals performing Natural visual based behaviors said Jason Kerr director of the Department of Behavior and brain organization Max Planck Institute for neurobiology of Behavior mpinb Bond Germany to stay up To date with latest top stories make Sure to subscribe to this YouTube Channel by clicking the button above This video in the study that is Published today in nature methods the Mpinb researchers developed a miniature
2 gram three Photon excitation Microscope which delivers a number of Firsts for the first time it is now Possible to image neural activity on a Single cell level from all cortical Layers without having to interfere with The animal's behavior made possible by a Remote focusing mechanism its modular Design also offers a high resolution Configuration which allows for Functional recordings from neuronal Samata and dendrites another important Feature is that due to a modified Detector system the microscope can be Used in fully lit conditions the Robustness of our new miniature Microscope to ambient light allows us to Image the brain's activity while the Animal is having access to its full Sensory repertoire we can now study Visually guided natural behavior like Prey hunting and Predator avoidance Alexander kliochnikov first author of The study to confirm range and stability Of the new miniature 3 Photon microscope The team imaged from the deep cortical Layers 4 for Lira n6 6 Lira while mice Were freely exploring an arena it turned Out that four Lira and six Lira neurons Were differentially modulated depending On the environmental light as the Microscope can easily be mounted again At the exact same position the same Neuronal populations can be imaged in
Follow-up session spread over days this Opens up the possibility to monitor Changes in the brain's activity for Example while the animal is learning Foreign [Music]
